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KMID : 0545119950050050250
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 5 p.250 ~ p.256
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Cloning and Sequencing of the ddh Gene involved in the Novel Pathway of Lysine Biosyntheis from Brevibacterium lactofermentum
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Kim, Ok Mi
Kim, Hyun Jeong/Kim, Sang Dal/Park, Dong Chul/Lee, Kap Rang
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Abstract
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The ddh gene encoding meso-diaminopimelate (meso-DAP)-dehydrogenase (DDH) in Brevibacterium lactofermentum was isolated by complementation of the Escherichia coli dapD mutation. It was supposed from subcloning experiments and complementation tests that the evidence for DDH activity appeared in about 2.5 kb Xhol fragmented genome. The 2.5 kb Xhol fragment containing the ddh gene was sequenced, and an open reading frame of 960 by encoding a polypeptide comprising 320 amino acids was found. Computer analysis indicated that the deduced amino acid of the B. lactofermentum ddh gene showed a high homology with that of the Corynebacterium glutamicum ddh gene.
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